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American Society of Microbiology ABMM Sample Questions:

1. A Gram-positive rod is isolated from the blood culture of a neonate with sepsis. The organism exhibits weak beta-hemolysis on sheep blood agar, is catalase-positive, hydrolyzes esculin in the presence of bile, and demonstrates characteristic "tumbling" motility in a wet mount prepared from a broth culture incubated at 25°C. Which organism is most likely?

A) Corynebacterium jeikeium
B) Erysipelothrix rhusiopathiae
C) Bacillus anthracis
D) Listeria monocytogenes

2. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:

A) Cryptosporidium parvum
B) Cyclospora cayetanensis
C) Entamoeba histolytica
D) Giardia lamblia

3. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate using broth microdilution. The lowest concentration of antibiotic that inhibits visible growth of the organism is reported as the:

A) Minimum bactericidal concentration (MBC)
B) Minimum inhibitory concentration (MIC)
C) Breakpoint
D) Zone diameter

4. Transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease (CJD) in humans and Bovine Spongiform Encephalopathy (BSE) in cattle, are caused by infectious agents composed primarily of:

A) Viroids (small, circular RNA molecules)
B) Slow-growing bacteria lacking a cell wall
C) Defective viral particles requiring a helper virus
D) Aberrantly folded host-encoded prion proteins (PrP<sup>Sc</sup>)

5. A patient with a history of neutropenia following chemotherapy develops a rapidly progressive pneumonia.
Bronchoalveolar lavage fluid reveals septate hyphae with acute angle (45-degree) branching. The MOST likely diagnosis is invasive pulmonary aspergillosis caused by:

A) Rhizopus species
B) Mucor species
C) Aspergillus fumigatus
D) Fusarium species

Solutions: