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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory is performing Gram staining on a bacterial smear. If the safranin counterstain is omitted, Gram-negative bacteria will appear:

A) Purple
B) Blue
C) Colorless
D) Brown

2. A Gram stain of cerebrospinal fluid (CSF) from a 2-year-old child with meningitis shows small, pleomorphic Gram-negative coccobacilli. Culture on chocolate agar yields growth, but there is no growth on standard 5% sheep blood agar unless streaked near a colony of Staphylococcus aureus. This phenomenon indicates a requirement for which growth factor(s)?

A) Factor V (NAD) only
B) Cysteine and pyridoxal
C) Both Factor X and Factor V
D) Factor X (hemin) only

3. A patient presents with a chronic, non-healing skin ulcer in a tropical region. Microscopic examination of a biopsy reveals amastigotes within macrophages. Culture of the biopsy on Novy-MacNeal-Nicolle (NNN) medium yields flagellated promastigotes. The MOST likely diagnosis is cutaneous leishmaniasis caused by:

A) Leishmania donovani
B) Trypanosoma brucei gambiense
C) Trypanosoma cruzi
D) Leishmania tropica or Leishmania major

4. A clinical laboratory performs a cefoxitin disk diffusion test on a Staphylococcus aureus isolate from a wound culture. The zone of inhibition measures 18 mm. According to current CLSI M100 guidelines, how should this result be interpreted regarding oxacillin/methicillin susceptibility?

A) Intermediate (requires MIC testing)
B) Indeterminate (requires mecA gene testing)
C) Resistant (report oxacillin as resistant; infer MRSA)
D) Susceptible (report oxacillin as susceptible)

5. A clinical laboratory receives a cerebrospinal fluid (CSF) sample from a 65-year-old patient presenting with fever, headache, and nuchal rigidity. Gram stain reveals Gram-positive cocci in pairs and short chains. Culture on blood agar grows alpha-hemolytic colonies that are optochin-susceptible and bile soluble. Which organism is most likely identified?

A) Streptococcus pneumoniae
B) Streptococcus agalactiae
C) Enterococcus faecalis
D) Staphylococcus aureus

Solutions: